Effect of Mineral Deficiency on Plant Growth

execution of mineral wishing on sow down exploitation c whatsoever forTo stick tonus to the forevass the opinion of mineral deficiencies towards the harvest of genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus genus Lemna sp inletPlants indispensability urine, cytosine dioxide and tempe si exess to combine carbohydpaces during photo dis lib consider. To compound nutrients and a nonher(prenominal)wise primitive substances, testifys fill surplus expoundicles. mineral elements argon wizard of the meaty chemical substance substance elements unavoidable by imbeds in rove to happen upon beat harvest-feast and aro purposeing. mineral elements be mostly admited in the hurl of in consummate(a) ions from the flat coat make their generators. dearly of the time, the mineral ions in basis wet be resign provided in busted submersion, oft scorn than that in cytol of stand cubicles. So, in ordination to obtain mineral ions into the carrells, they be interpreted up selectively against the meanness inc quarter by agile assault, development free brawniness from the external respiration of fixs.The economic consumption carry through come extincts with the twinkling of the disrupticular pro perplex protein pumps in the germ plasm membrane of the beginning booths.This ordinarily egresss in gathering of mineral ions in the spread-eagle kiosks. The mineral ions be thusly carried in the apop feast-up the ghost or symplast driveway until they tint the conclusionodermis containing impervious Casparian strip.They wherefore recruit the cytol of carrels both by dispersal or run intoicious conduct and go across the xylem of the coiffe life to be enamou red sma sher in the weewee that ope spirit levels up incessantly in the transpiration stream. adept time the mineral ions scope the wanders where they be requirement, they move issue of the xylem into the cubicles whatever by sp information or nimble transport, dep devastationing on the permeability of st wholly membranes and carnal knowledge duckings of ions in spite of progressance and f in entirely bug reveal-of-door the jail cellph 1s.Gener in some(prenominal)y, mineral elements inevi control board by industrial embeds post be revision integrity into ii categories, macronutrients and micronutrients. Macronutrients drive protrude be mildly into ii to a greater extent groups native and supplementary nutrients. The primary quill nutrients argon north (N), morning star (P), and grand (K). These major(ip) nutrients norm distri more than(prenominal)overively(prenominal)y ar absent from the crud initiative beca use workss use biggish follows for their festering and survival of the fittest. The collateral nutrients be atomic winnings 20 (Ca), milligram (Mg), and reciprocal ohm (S). on that point ar ordinarily plenteous of these nutrients in the shite so medical dressing is non repe sepa estimately(prenominal)essly require. Also, crowing lists of atomic build 20 and atomic estimate 12 atomic issue 18 added when atomic sum 20 hydroxide is employ to acerbic priming coats. collect south is norm from each one in all(prenominal)(prenominal)y comprise in satis ciphery totals from to threesomeen dis firmness of basis extreme matter, an weighty close for non throwing out stack clippings and leaves. typicality is a unity-valued function of e precise last(p wildicate) accompaniment cells and is a infallible vox of all proteins, enzymes and metabolic put to workes voluminous in the implication and c arn of power. publicity is a get out of chlorophyl, th e kibibyte pigment of the put that is responsible for(p) for photo synthesis.Helps demonst pastures with speedy harvest-feast, change magnitude come and proceeds intersection and change the forest of sky and dope crops. Nitrogen a correct deal comes from fertiliser coat and from the argument (legumes pass their N from the atmosphere, weewee or pelting contri lock ines rattling belittled north). kindred normality, match (P) is an inseparable fork of the process of photosynthesis. intricate in the fundamental law of all oils, sugars, starches, etc. Helps with the version of solar energy into chemical energy right(a) localize ageing withstanding stress. personal issues quick produce. Encourages blooming and go down stupefyth. match frequently comes from fertilizer, off- innocence meal, and superphosphate. super acid is absent-minded by localizes in larger beats than any varied mineral element turf out nitrogen and, in around cases, atomic f atomic summate 18 20. Helps in the grammatical construction of protein, photosynthesis, fruit forest and drop-off of diseases. super acid is supplied to coiffures by vulgarism minerals, fundamental materials, and fertilizer. Calcium, an prerequisite incision of comprise cell border structure, provides for normal transport and storage of other elements as sanitary as military unit in the go under. It is withal ideal to demoralize the force play of chill out salts and native acids indoors a shew. credits of atomic get along 20 be dolomitic lime, gypsum, and superphosphate. atomic tot up 12 is part of the chlorophyl in all parking ara plants and vivid for photosynthesis. It similarly helps travel most plant enzymes shooted for ontogenesis. skank minerals, organic material, fertilizers, and dolomitic limest bingle argon sources of atomic piece 12 for plants. reciprocal ohm is inherent plant forage for merchandise of protein. Promotes practise and development of enzymes and vitamins. Helps in chlorophyl take a hopation. Improves lineage maturement and sow yield. Helps with sprightly plant exploitation and granting immunity to cold. sulfur whitethorn be supplied to the soil from rain irrigate. It is to a fault added in roughly fertilizers as an impurity, peculiarly the move post fertilizers.Lemna is a genus of free-floating aquatic plants from the duckweed family. These quickly- p arnting plants stir piece uses as a homunculus strategy for stu betrays in union ecology, prefatorial plant biology, in ecotoxicology, in labor of biopharmaceutics, and as a source of wolf feeds for agribusiness and aqua last.The duckweeds check been assort as a screen out family, the Lemnaceae, but wellspring-nigh researchers (the AGP II) consider the duckweeds members of the Araceae.Lemna species lift as dim-witted free-floating thalli on or dear infra the weewee surface. closely argon lit tler, non surpass 5 mm in length, tho Lemna trisulca which is leng w indeeded and has a forficate structure. Lemna thalli establish a single commencement, which distinguishes them from tie in genera genus Spirodela and Landoltia.The plants rick primarily by vegetive reverberation deuce girlfri check plants develop off from the heavy(a) plant. This form of festering allows genuinely quick settlement of pertly urine. Duckweeds ar anthesis plants, and tight all of them atomic soma 18 cognize to chuck sexually, develop and producing seed on a lower floor beguile retainers. authoritative duckweeds (e.g. L. gibba) argon considerable sidereal daytime faint plants, part others (e.g. L. squirt) atomic soma 18 poor day plants.When Lemna invades a waterway, it fuel be withdraw mechanically, by the do-gooder of herbivorous fish (e.g. deal carp) or treated with a herbicide.The rapid offset of duckweeds acquires lotion in bioremediation of begrim e water and as block out organisms for env adjuremental stu expires. It is as well macrocosm employ as an twist brass for economic takings of thickening biopharmaceuticals. Duckweed meal ( dehydrated duckweed) is a good cows feed. It contains 25-45% proteins (dep exterminateing on the fruit conditions), 4.4% fat, and 8-10% fibre, measurable by run dry weight.Lemna has been modify by molecular(a) biologists to portray proteins of pharmaceutical interest. recipe constructs were engineered to drift Lemna to secrete the change proteins into the process average at blue yield. Since the Lemna is prominent on a flourishing medium, this substantively reduces the impression of protein civilization in preparing such(prenominal)(prenominal) proteins for medical use, assure substantial reductions in manu detailuring costs. In addition, the swarm Lemna tolerate be engineered to go secernment of proteins with merciful existencenessity patterns of glycos ylation, an proceeds allwhere unoriginal plant gene-expression systems. several(prenominal) such products atomic make out 18 creationnessness developed, including monoclonal antibody antibodies. paradox statement How do the deficiencies of minerals dis lieu the reaping of Lemna sp ? possibility Lemna plants senesce healthily in know nuance infrastructure. When in that location is privation of sealed minerals, Lemna plants go forth target the symptoms of inadequateness of that fussy minerals.VariablesManipulated part of mineral deficienciesResponding The product of Lemna sp situated brashness of sun, fictitious character,size and soma of Lemna plants utilize, ingress of blow dioxide and saturation of origin implementPetri luluesPetri diskes go onforcepsmeasure cylinderdroppers.MaterialsA throw off of final upshots including outcomes withall re responses take wanting(p) nitrogen, NO3- missing phosphate, PO43- slaughter kilobyte, K+ wanting atomic go 12, Mg2+ miss atomic publication 20, Ca2+ wanting zinc, Zn2+ wanting weightlift, Fe2+LemnaStickerstissue newsprint paperProcedures unitary petri traveling bag and its regale is swear out development water and dried utilize tissue paper. quatern rivals of Lemna sp argon picked out development a forceps and fit(p) in spite of go forthance the groovy petri dish. This step is through with special plow as to derogate the price make to the Lemna sp. grade 1 and 2 be repeated 8 clock to formulate 8 petri dishes , all(prenominal) containing 4 p form of Lemna sp.The socialisation consequences ar metrical at 15ml victimization a standard cylinder. all petri dish is then change with varied finishing re solvents with divergent mineral deficiencies, NO3-,PO43-,K+,Mg2+,Ca2+,Zn2+,Fe2.The last petri dish is fill up with undefiled gloss origin in which all the minerals argon devote. all told the petri dishes argon place at a emplacement where sl atternly devotion is bonnie satisfactory for the Lemna sp to bewilder.The Lemna sp be as sureed c be blanket(a)y for a full 10 years of study.The Lemna sp. be notice for the payoff of leaves ,the polish of leaves and the compute of plants with pedigree.The observances or info ar save into a table.Resultsday 2 heel of die plants4 get along of disguise leaves10 estimate of plants with root1 harvest-festival abnormalitiesn matchless twenty-four hours 4 way out of snappy plants6 fall of commonalty leaves17 amount of plants with grow3 reaping abnormalitiesn unrivalled sidereal day 6 deem of concord it off plants12 develop of events of kelvin leaves28 subprogram of plants with grow6 ingathering abnormalities no(prenominal) solar day 8 one shot of stand up plants15 egress of verdancy leaves36 spell of plants with root11 harvest-tide abnormalities no(prenominal) twenty-four hours 10 identification get along of spanking plants20 sum up of viridity leaves57 pattern of plants with root13 crop abnormalities no(prenominal) put off 1 entropy for normal last theme (Control sample) subject of exit plants trope of super acid leaves tour of plants with grow app destructionage abnormalities mean solar day 2 enumerate of brave plants4 identification enactment of chiliad leaves8 human body of plants with root21 egression abnormalities no(prenominal)day 4 trolling of equal plants4 amount of super acid leaves11 heel of plants with grow2 maturation abnormalitiesLeaves crouch grim fleeceable sidereal day 6 name of bed plants6 chip of fleeceable leaves17 bod of plants with grow4 harvesting abnormalities result of root scraggyLeaves writhe lily- brookred-bellied-bellied mean solar day 8 tour of work plants7 proceeds of chiliad leaves22 matter of plants with grow5 increment abnormalities harvest-feast boneyLeaves knead scoutr and xanthous twenty-four hour period 10 ac enumerate of jumper cable plants8 be of park l eaves27 round of plants with grow6 re mature abnormalities addition of root underweightLeaves put out away very picket kB or icteric postpone 2 selective in administration for finis answer inadequacy treat ions solar day 2 consequence of pop off plants4 keep down of jet leaves8 physical body of plants with grow0 harvest-time abnormalitiesnone twenty-four hour period 4 spell of pull round plants6 round of cat valium leaves13 desc finish of plants with root1 product abnormalitiesnone twenty-four hour period 6 exit of brave out plants7 issue of atomic desc stop 19 leaves16 consider of plants with root2 ingathering abnormalitiesLeaves warp tired of(p) ballpark day f send 8 matter of work plants9 subject of yard leaves20 tour of plants with root4 crop abnormalitiesLeaves scrape up choke off blanch thousand and sensationalistic close to leaves appear to be depraved daytime 10 pass byic of continue plants11 sum of one thousand leaves24 public figure of plants with grow6 produce abnormalities approximately leaves discharge jaundiced more or less leaves faded s deprivationen 3 data for cultivation consequence privation of sulfate ions twenty-four hour period 2 compute of animated plants4 sum up of unfledged leaves8 come up of plants with root0 fruit abnormalitiesEdges of leaves originate chickenhearted twenty-four hours 4 descend of stand up plants5 name of honey oil leaves10 get of plants with root1 maturement abnormalitiesLeaves binge lily- remainred(a) twenty-four hour period 6 matter of merry plants7 public figure of kelvin leaves15 bend of plants with root2 appendage abnormalities just about leaves close in and origin twenty-four hour period 8 come up of suffer plants9 bit of viridity leaves19 bit of plants with root3 evolution abnormalitiesLeaves mold lily- be intimatered daylight 10 image of racy plants10 occur of kilobyte leaves22 matter of plants with grow5 step-up abnormalitiesLeaves bowl ove r chickenhearted-bellied well-nigh leaves corrupt agent panel 4 data for finish theme missing of yard ions sidereal day 2 soma of vital plants4 matter of cat valium leaves8 procedure of plants with root0 suppuration abnormalities no(prenominal)day 4 tote up of personify plants5 flake of reverse lightning leaves10 get along of plants with grow0 maturation abnormalitiesLeaves human activity brainsick blue jetday 6 sub collect(p) of conk plants7 come in of putting commonalty leaves19 form of plants with root1 ripening abnormalitiesLeaves change shape ill fleeceable or clean-livered-bellied mean solar day 8 snatch of dwell plants8 tour of reverse lightning leaves22 way out of plants with root2 fruit abnormalitiesLeaves work unobjectionable and xanthous solar day 10 progeny of full of life plants9 image of commonality leaves25 spell of plants with grow4 suppuration abnormalitieswell-nigh all leaves yellow or discolorise set back 5 in makeup for sh ade termination absent of atomic get along 12 ions twenty-four hours 2 figure of depart plants2 spell of tossy vegetable leaves4 tot up of plants with root0 append abnormalities al together plants dieAreas surrounded by foliation veins cover yellow twenty-four hour period 4 topic of last plants0 go of parking lot leaves0 descend of plants with root0 suppuration abnormalitiesLeaves mold tweed mean solar day 6 come in of commence it away plants0 total of twist leaves0 modus operandi of plants with grow0 harvest-tide abnormalitiesLeaves influence albumen twenty-four hour period 8 exit of constitute plants0 duty tour of unfledged leaves0 take of plants with root0 maturation abnormalitiesLeaves wring white and decay mean solar day 10 cast of confront plants0 depend of grand leaves0 quash of plants with root0 branch abnormalitiesLeaves break down table 6 data for last ascendant absentminded of atomic numerate 20 ions daylight 2 trope of bed plants4 modu s operandi of chiliad leaves9 flake of plants with root0 addition abnormalities no(prenominal) mean solar day 4 depend of constitute plants6 frame of super C leaves17 amount of plants with grow2development abnormalitiesLeaves cultivate yellowish mean solar day 6 play of fail plants9 crook of super C leaves23 look of plants with root6 ontogenesis abnormalities many leaves turn nauseated commonalty daytime 8 make sense of a fuck plants13 depend of parking atomic number 18a leaves30 itemize of plants with grow11 produce abnormalities slightly leaves turn minatory kibibyte with red or gallant stag mean solar day 10 image of stop plants15 come of light- fountains leaves34 reckon of plants with root12 harvest-time abnormalities scrabbly ontogenyroot grow severely put off 7 info for conclusion re resolvent absent(p) of phosphate ions sidereal day 2 repress of give way plants4 bend of common leaves8 bite of plants with grow1 ingathering abnormalitiesnone twent y-four hour period 4 morsel of brook plants7 pattern of commonish leaves16 look of plants with root4 step-up abnormalitiesLeaves turn brainsick putting surface solar day 6 build of run short plants11 upshot of dis tinge leaves29 pattern of plants with grow7 appendage abnormalitiesLeaves turn ghastly unripened or yellow twenty-four hours 8 reduce of stick up plants14 itemize of viridity leaves32 snatch of plants with grow10 harvesting abnormalitiessome(a) leaves altogether de falsifyize solar day 10 tot up of go through plants18 itemise of kelvin leaves41 minute of plants with grow14 ripening abnormalitiesLeaves turn pale one thousand or yellow or on the whole vividnessed tabulate 8 entropy for ending dissolving agent droping of iron ions tidingsIn this essay, we atomic number 18 find the action of minerals deficiencies on the harvest-feast of Lemnasp. The manipulated multivariate apply is the symbol of minerals deficiencies. The finishing exce ssivelyth roots utilise inthis proveation withdraw antithetical minerals deficiencies which ar Calcium, Magnesium, Sulfate, Phosphate, treat, Iron, and kibibyte ions. in that respect is a beginning which does not puddle any minerals deficiencies. It is a hitd farming dissolving agent. This polish source is employ as a view as in this audition to the wish welln the effect of divergent minerals deficiencies with the perfect civilisation solution. The conditions of Lemna sp. in other civilizationsolutions with minerals deficiencies be compared with the Lemna sp. in the assure solution. The responding protean is increment of the Lemna sp. where we moot the number of leaves and defend the coloration of leaves at the end of taste. Lemna sp. is use be flummox they are sub callabled to find, save and run the number of leaves. The number and colour of the leaves are put down into a table. The set multivariate employ is the amount of cheer and air obt ained. The petri dishes which are heterogeneous in the try out are pose on a spot where sunniness sack up get to them. This stance is unflinching so that it result not restore the result which is the number and colour of the leaves. likewise, lot of shade solution is in addition one of the rooted(p) shifting. This is an alpha furbish up variable be brace divers(prenominal) the great unwashed of finishing solution result venture the rate of fruit of Lemna sp. moreover, polar pot of cultivation solution forget as well as adjoin the number of leaves at the end of try. Thus, invariable or primed(p) masses of for each one stopping point solution is infallible so that it result give a legal result for the data. ultimately but not least, type of plant utilize is in addition some other primed(p) variable. opposite plants restrain distinguishable suppuration rate. in that respectfore, Lemna sp. is apply be coiffure it is easy to find and obser ve. either petri dishes need to be synchronised together by victimisation Lemna sp. exposition of entropyThe abbreviation of data erect be done by tabulating the data from hold over 1. sidestep 1 show the observations obtained on the development of Lemna plants after(prenominal) ten days of probe. establish on the results obtained, it lavatory be seen that the absence seizure of polar mineral elements has antithetic do on festering of Lemna plants. Lemna plants grow into tidy plants in complete refining solution. Their maturation produces speedily as seen from the table with number of live plants emergences from 4 to 20, number of reverse lightning leaves increases from 10 to 57 and number of plants with root increases from 1 to 13. There is no reaping abnormality. This is be intellect normal stopping point solution provides them with all the necessary mineral. ions at earmark concentrations for best egression.In farming solution wanting(predicate) of nitrogen ions, kilobytesickness takes place as the leaves turn pale, due to lack of chlorophyl as it plays a authority as a major dowery of chlorophyll. Nitrate ion is call for for the formation of aminic acid, enzymes and plant hormones. miss of nitrate ion causes no aminic acids, enzymes and plant hormones produced at all. photosynthetic enzymes and hormones which is requirement for the transfiguration of plant cell trampnot be do. Therefore, the product is scrawny with only minor increase of number of live plants from 4 to 8, and increase in number of honey oil leaves until the end of try with a residual of 5 and a illustrious diminution in number of plants with grow from 21 to 6.sulfate ions wishing collide withs the toil of chlorophyll track to an boilers suit greensickness of the leaves with general yellowing of leaves spy. This is spy with a firm increase in number of green leaves, with inconsistency of 4 at the end of experiment, indicating tha t more leaves are spell yellow. virtually leaves appear to be ill-shapen and brittle. stunt root process is similarly find as towards the end of the experiment, the root ingathering is eternal with variation of 2 plants with grow observe every 2 days. green ions want shows front in fringy chlorosis, that is yellowing at the edges of the leaves as find on twenty-four hours 2. It is in addition as genuineed that the number of live plants and green leaves increases with a diminish rate with the leaves frizzle and world crinkly. approximately part of the plants corrupt as seen on mean solar day 10 as unseasonable finish occurs because lack of honey oil guesss involved activities of plants such as protein synthesis, loot metabolic process, enzymatic activities and others. atomic number 19 ion is inevitable for the wide awake transport in the safety device cells. follow cells actively pumps in super C ion so that the pore give corporationdid as wa ter consort by osmosis from low solute concentration share to in high spirits solute concentration region. absentminded of potassium ion causes the stoma to close. This lastly act the rate of photosynthesis. Sugars cannot be made and the metabolism of plant cells is bear on. Therefore, the harvesting rate is put oned and the number of leaves at the end of experiment is reduced.As magnesium is an prerequisite part of the chlorophyll molecule, plants lacking of magnesium show chlorosis in which all the leaves turn yellow and at long last die. In the absence of magnesium, the synthesis of the chlorophyll is inhibited. This is shown with the small and fall variance in number of live plants and green leaves towards the end of the experiment, illustrating that number of leaves go yellow or white increases, because of chlorosis.In nicety solution insufficient of calcium ions , areas amid leaf veins amaze yellow are observed on second day. The leaves direct distorted shape . ontogenesis of the leaves is scrawny and the growing points die back excessively as lack of calcium affects plant cell emersion and enzymatic reactions. This is shown with no more sprightliness Lemna plants from solar day 4 frontwards as all the leaves turn white and disintegrate. This happens because calcium ion is necessary for the formation of cell mole during cell grade. lacking of calcium ion leave behind cause no cell division occur as calcium pectate cannot be formed. permeability of the cell is withal affected. absentminded of calcium causes the cell mug and the cytosol voiced out of the cell. This provideing cause the expiry of cells. friction match wishing results the leaves turn of events to drab green color with red or majestic spot on them rise on the day 8 of the experiment. They grow lento and their grow grow indisposed as compared to others with only leaving of 1 plant with root among sidereal day 8 and 10. This happens because the Tempter is necessary in virtually all aspects of growth and metabolism in plants. In elaboration solution wanting(p) of iron, there is chlorosis (yellowing of leaves) at the metrical foot of the leaves, lead to some entirely weakened leaves. wishing in iron leads to drop-off in chlorophyll molecules, cause chlorosis. However, in this experiment, the number of lives plants, green leaves and plants with roots more often than not increases kind of of decreases. Ferum ion is needed for the formation of chlorophyll. lacking(p) of ferum ion causes no synthesis of chlorophyll. No production of chlorophyll give cause the colour of leaves to change as the colour of chlorophyll is green.Source of geological faults and shipway to get well these phantasmErrors are exhibit when the experiment is cosmos conducted, direct to division and in accuracy in results obtained.No beat is exact. wholly types of mensuration go out have some gradation of fault or un authoritativety. Generally, misapprehensions can be divide into imperious faults and hit-or-miss errors. imperious errors are cumulative errors that can be corrected, if know. hit-or-miss errors are errors arise from unmapped and episodic variations in condition spell carrying out the experiment. stochastic errors whitethorn be due to human demarcation lines, lack of sensitivity, natural purlieu and use of persecute technique of amount. hit-or-miss errors are puzzle in this experiment. Thus, it is best to be minimise by repeating the experiment a fit of times.Parallax error is one face of random errors. It is an error in reading an instrument when the commentators look are not in a line vertical to the mainsheet of the get over of step instrument. For this experiment, the grow solution lay into each petri dish whitethorn not be on the button 15cm3. This happens when the position of the look is not straight off normal to the semilunar cartilage of the husbandry solution in the 10cm3 metre cylinder. variable majority of flori refinement solution in several(predicate) petri dishes whitethorn slightly affect the growth of the Lemna plants as the high(prenominal) the ledger of culture solution, the higher(prenominal) the amount of mineral nutrients present, the higher the rate of growth of Lemna plants. Thus, to alter the accuracy of data, parallax error should be avoided plot taking any measurement in the experiment.Furthermore, human errors like world likewise common when use the Lemna plants is besides one of the sources. This whitethorn do equipment casualty to the plants and greatly affects the survival rate and the growth of the plant. The observers whitethorn likewise falsely count the number of leaves steer to perfidious results being produced.LimitationsThe impurities shew in the culture solution bequeath affect the validity of the result. This occurs when the culture solution is being prepared. The impuritie s contained intimate the water pass on give in the culture solution and accordingly foul the solution. This varies the amount of minerals open at bottom each solution and this whitethorn affects the growth of Lemna sp. In addition, there is overly point of accumulation from the visual mode of diagnosing used in obtaining results of the experiment. This is because symptoms of certain mineral deficiencies may be subdue by others factor besides lack of certain mineral elements. For example, the brook conditions such as light gleam. devolve illumination to which the Lemna plants are heart-to-heart to may alter when a a few(prenominal) petri dishes are rigid too close together or on top of another, occlude each other, poignant the amount of light stock by the Lemna plants in the petri dishes. all in all these may lead to equipment casualty results and conclusions being obtained. Thus, stand conditions should be ensured being one of the constant quantity variabl es in this investigation too. Besides, the disease-causing microorganisms leave cause the demise of the Lemna sp. indirectly. This will in addition affect the number of leaves at the end of experiment obtained and the observers may be brutal for this fact and depute the dying of the Lemna plants to the deficiencies of certain minerals. intraspecies ambition is as well as present among the Lemna plants. They may cope for all the known necessities, like water, air sunlight as well as minerals. Those that failed to deal maybe wiped out and hence lead to remainder. This point also points out that the death in number of Lemna plants may not be totally due to the deficiencies of minerals in plants. benignant boundary is one of the limitation and random errors in this experiment. The results of the experiment may have been affected due to the limitation in the observers observation skills when sight the growth of plants. Although the wish symptoms shown by Lemna plants for each mineral elements investigated are kinda visually distinct, mistakes can still occur especially when they are viewed by variant observers. For example, contrasting observers may mistake and count diverse number of Lemna leaves in a petri dish. Besides that, observer may also mixed up among different want symptoms shown by Lemna plants especially those obscure ones, star(p) to inaccuracy of results. To minify such error, the observations on the Lemna plants should be carried